Additional file 3: Figure S3.

Autophagy is not directly involved on toxicity of the cotreatment of Rsv and TMZ. (a) Representative images of U87 human GBM cells transfected with the plasmid pRGFP-LC3 and treated with Rsv 30 μM, TMZ 100 μM or R30+T100 for 48 h; white arrows: cytosolic green dots representing LC3-GFP marked autophagosomes; scale bar: 10 μm; (b) percentage of cells that presented more than five well-defined cytosolic green dots, for each treatment; a p<0.01 and b p<0.001 in relation to control; (c) cells were treated as in (A), marked with AO and the percentage of cells positively marked to acridine orange (i.e. red marked cells) were evaluated by flow cytometry after 4, 24 and 48 h; a p<0.05 and b p<0.01 in relation to control.

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Filippi-Chiela et al. BMC Cancer 2013 13:147   doi:10.1186/1471-2407-13-147