Additional file 2: Figure S2.

Apoptosis or ROS are not involved in RT toxicity. U87 cells were treated with Rsv 30 μM, TMZ 100 μM or RT for 48 h. After this, cells were (a) stained with 6 μM PI, to evaluate the membrane integrity and induction of necrosis - numbers indicate the percentage of positively marked cells (ratio of PI labeled cells/total cells) for at least 100 cells counted per treatment; scale bar: 100 μm; detail shows the morphology of treated cells. (b) Cells were treated as in (a) and marked with annexin V-FLUOS/PI and evaluated by flow cytometry. Numbers in quadrants represents the percentage of cells ± SEM of three independent experiments; (c) Cells were treated as in (a) and marked with DCFH to measure reactive oxidative species followed by flow cytometry. Left -% in relation to control of DCFH intensity; right – graphs of DCFH staining from Guava Software; n=2.

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Filippi-Chiela et al. BMC Cancer 2013 13:147   doi:10.1186/1471-2407-13-147