Additional file 1: Figure S1.

Autophagy and toxicity in other glioma cell. (a) U87, U138 or U251 cells were treated with Rsv 30 μM, TMZ 100 μM or RT for 48 h a, followed by cell number determination (upper panel) and marking with AO followed by flow cytometry to determine the percentage of AO-positive cells (lower panel); (b) primary glioblastoma cells were treated as above and analyzed for cell number and AO staining after 48 h Numbers in the quadrants refer to average of events (black) or X-mean of AO red fluorescence intensity (red) ± SEM of three independent experiments; * p<0.05, ** p<0.01.

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Filippi-Chiela et al. BMC Cancer 2013 13:147   doi:10.1186/1471-2407-13-147