Photomicrographs. Representative frozen tissue was collected at the time of surgery, sections were stained with hematoxylin and eosin and DNA was extracted from the frozen tumors. Because the frozen sections were quite thick, they have not photographed well. We present here images of the paraffin-embedded tumors that reflect the frozen sections that were used for DNA extraction. The poorly differentiated original tumor appeared to be arising from the right ovary; A - solid proliferation of highly atypical epithelial cells with enlarged, pleomorphic nuclei and macronucleoli. H&E X400; metastases were widespread, and a biopsy was taken from the omentum; B - solid sheet of malignant cells displaying the same microscopic features as the primary ovarian carcinoma. The tumor cells invade the adjacent fibrofatty tissue of the omentum. H&E X400. Despite only minimal residual disease being present at the end of the primary surgical resection, the tumor clinically recurred after only three months of chemotherapy (discussed above) and at laparotomy, tumor was found on the surfaces of pelvic and abdominal organs and was biopsied: C - the malignant cells are smaller than the primary ovarian and omental carcinoma cells. They have clear, cytoplasmic and smudgy nuclear substance, and occasional giant macronuclei and nucleoli. These features may be a reflection of degenerative effects of previous chemotherapy. H&E X400.
Zhang et al. BMC Cancer 2013 13:146 doi:10.1186/1471-2407-13-146