Open Access Research article

The combination of ANT2 shRNA and hNIS radioiodine gene therapy increases CTL cytotoxic activity through the phenotypic modulation of cancer cells: combination treatment with ANT2 shRNA and I-131

Yun Choi1, Ho Won Lee2, Jaetae Lee23 and Yong Hyun Jeon23*

Author Affiliations

1 Department of Pathology, Seoul National University College of Medicine, Seoul, Korea

2 Department of Nuclear Medicine, Kyungpook National University, 807 Hogukro, Bukgu, Daegu, 700-721, Republic of Korea

3 Leading-edge Research Center for Drug Discovery and Development for Diabetes and Metabolic Disease, Kyungpook National University, 807 Hogukro, Bukgu, Daegu, 700-721, Republic of Korea

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BMC Cancer 2013, 13:143  doi:10.1186/1471-2407-13-143

Published: 22 March 2013

Additional files

Additional file 1: Figure S1:

In vitro caspase-3 activity after treatment with ANT2 shRNA, I-131 and combination treatment. Detailed experimental procedures are described in the methods section. At 2 days after treatments, cells were lysed and an equal volume of a proluminescent substrate (DEVD), and cytosolic proteins were added to a white-walled 96-well plate and incubated at room temperature for 1 hour. The luminescence of each sample run in triplicate was measured in a plate-reading luminometer. The columns indicate the mean of triplicate experiments; the bars indicate the SD.

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Additional file 2: Figure S2:

Tumor volume and weight measurements. (A) The tumor volume was defined as V = 1/2(L × W2), where L is length (longest dimension) and W is width (shortest dimension). (B) The tumor mass was extracted and weighed 35 days post-tumor challenge. The data shown are the mean of triplicate experiments; the bars represent the mean ± SD; n = 7 mice/group.

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