Influence of αB-crystallin knock-down on VEGF secretion. Knockdown of αB-crystallin mRNA expression in UT-SCC-5 cells by two different siRNAs, αB1 and αB2, compared to negative control siRNA LUC (Luciferase) as measured by quantitative PCR (a). VEGF secretion levels in UT-SCC-5 cells transfected with the αB1, αB2 or LUC siRNA under normoxic and hypoxic conditions determined by ELISA (b). Statistical analysis was performed using One-way ANOVA and Tukey’s Multiple Comparison Test. *** P < 0.001, ** 0.001 < P < 0.01, * 0.01 < P < 0.05; a.u: absorbance units.
van de Schootbrugge et al. BMC Cancer 2013 13:128 doi:10.1186/1471-2407-13-128