TST measurements of chimeric integrin-expressing aggregates of MLL cells. Comparison of aggregate cohesion by ANOVA and Tukey's MCT shows that aggregates of MLL-X5C5 cells are significantly more cohesive than those of the MLL parent line (p < 0.001) and MLL-X5C2 (p < 0.001). No difference in cohesion was detected between MLL and MLL-X5C2 (p > 0.05) (A). In vitro invasion of chimeric integrin-expressing MLL cells. The number of cells able to detach from n = 10 aggregates composed of MLL, MLL-X5C2, and MLL-X5C5, and to invade through a Matrigel filter was compared. Whereas no difference in invasiveness could be observed between MLL and MLL-X5C2 (p > 0.05), MLL-X5C5 cells were significantly less invasive than aggregates of either parent MLL (p < 0.001) or MLL-X5C2 (p < 0.001) (B). Representative images of invading cells are included as insets.
Jia et al. BMC Cancer 2012 12:94 doi:10.1186/1471-2407-12-94