Effect of Rapamycin, NVP-BEZ235 and PP242 on the growth of LS174T and SW480 tumor xenografts. A: An equal amount of LS174T (left panel) or SW480 (right panel) cells were harvested and injected subcutaneously into nude mice. Once the tumor reached 25 mm3 mice were randomized into four groups and treatments were started with vehicle (Control), rapamycin (R, 1.5 mg/kg/day), NVP-BEZ235 (N, 30 mg/kg/day) or PP242 (P, 60 mg/kg/day). Five mice were included in each group. Tumor volumes were evaluated using caliper measurements and calculated with the formula V = π/6 × a2 × b where a is the short axis and b the long axis of the tumor. Points, mean value of tumor volume; bars, SD. B: After 20 days of treatment, mice were sacrificed, tumor xenografts were harvested and tumor weight was measured. Columns, mean tumor weight (five tumor xenografts in each group); bars, SD. *, P < 0.05, compared to control. C: Tumor xenografts were lysed and analyzed by Western blot for phospho-S6 ribosomal protein, total S6 ribosomal protein, phospho-Akt and total Akt. The illustrated blots are representative of three independent experiments.
Blaser et al. BMC Cancer 2012 12:86 doi:10.1186/1471-2407-12-86