Additional file 2.
Figure S2. Effect of the expression of Y477F ezrin on primary tumor growth: Mice injected in the mammary fat pad with AC2M2 cell clones (pCB6, A43, C13) in Figure 2 were monitored every two days, and palpable tumors were measured using Vernier calipers. Primary tumors were excised after 21-23 days, and mice were allowed to live for 40 days (20 days post-resection of the primary tumor). Panel A) Equal protein amounts of primary tumor tissue lysates were subjected to SDS-PAGE. Western blotting was performed using antibodies against VSVG, ezrin and γ-tubulin. Mean optical density ratios of ezrin vs γtubulin bands for the three tumor groups (0.52, 0.55 and 0. 64) showed no significant difference, most likely due to host tissue contribution to the total ezrin pool. Panel B) Primary tumor volumes in each group were plotted as a function of days post engraftment. The mean +/- one standard deviation (bars) is indicated for each group (pCB6, A43, C13) at each time point. The natural log of the tumor volumes as measured on days 15, 17, 20, and 23 was compared among the groups by a linear mixed effect model as estimated by restricted maximum likelihood using the SAS MIXED procedure (SAS Institute Inc., 2008). A first order autoregressive correlation structure was used to account for within mouse dependence . No significance among groups on all four days was detected, using a global F-test (overall p = 0.57).
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Mak et al. BMC Cancer 2012 12:82 doi:10.1186/1471-2407-12-82