Additional file 4.
Figure S4. Quantitative real-time PCR measure of the mRNA expression of stem/progenitor markers in Daoy cells. (A) Daoy cells were cultured under standard conditions or serum-free conditions. Stem/progenitor cell markers increase when cells are grown under serum-free tumor sphere conditions compared to standard serum containing adherent monolayer conditions. (B) Knockdown of PLK1 mRNA with shPLK1 1325 decreases SOX2 mRNA in Daoy cells grown as tumor spheres. There was no significant change in Nanog, NES, or c-Myc mRNA.
Format: JPEG Size: 420KB Download file
Harris et al. BMC Cancer 2012 12:80 doi:10.1186/1471-2407-12-80