Table 1

Enumeration and genomic profiling of circulating tumor cells (CTCs) from 20 castration resistant prostate cancer patients

Patient No.

CTC/7.5 mL

IE/FACS

Days from draw to IE/FACS

No. of cells collected as input for WGA

aCGH


1

1

n.d.

N.A.

N.A.

N.A.

2

1

n.d.

N.A.

N.A.

N.A.

3

85

Yes

1

50

Yes

4

12

Insufficient sample

N.A.

N.A.

N.A.

5

144

Failed due to machine error

1

N.A.

N.A.

6

6

Insufficient sample

N.A.

N.A.

N.A.

7

10

Insufficient sample

N.A.

N.A.

N.A.

8

2

n.d.

N.A.

N.A.

N.A.

9

590

Yes

1

20 × 2

Yes (duplicate)

10

151

Yes

4

20

Yes

11

9

Yes

2

20

Yes

12

2

n.d.

N.A.

N.A.

N.A.

13

55

Yes

1

20

Yes

14

590

Yes

2

20

Yes

15

42

Yes

2

33

WGA failed

16

0

n.d.

N.A.

N.A.

N.A.

17*

54

Yes

1

20

Yes

18

33

Yes

1

20

Yes

19

34

Yes

2

20

Hybridization failed

20*

12

Yes

1

18

Yes


*Patients with available matched primary tumor

IE/FACS-immunomagnetic enrichment and fluorescence activated cell sorting; aCGH-array comparative genomic hybridization; WGA-whole genome amplification; n.d.-not done; N.A.-not applicable

Magbanua et al. BMC Cancer 2012 12:78   doi:10.1186/1471-2407-12-78

Open Data