Additional file 4.
Figure S1. Preparation of fully methylated and unmethylated controls. The methyltransferase (Sss I) treatment of 1 μg DNA was performed at 37°C for 4 h. The fully unmethylated control was construct by PCR with the following primers (forward primer 1: 5'-CATTATCCAGCCCTCTTATTTCTC-3' and reverse primer 2: 5'-ACTTCTCTACAGGACATTTGCTTG-3') using 20 ng of DNA as template. Then, these DNA samples were applied to bisulfite treatment.
Format: PDF Size: 202KB Download file
This file can be viewed with: Adobe Acrobat Reader
Yokoyama et al. BMC Cancer 2012 12:67 doi:10.1186/1471-2407-12-67