MSE analysis of DNA methylation status of MUC1, MUC2, MUC3, MUC4, MUC5AC and MUC17 promoter regions in 12 cell lines. The results of each mRNA analysis using RT-PCR are shown in the upper row of MSE results. The expression level of GAPDH mRNA in each cell line was used as a control in the RT-PCR analysis. The methylation pattern of each gene was consistent with the results of massARRAY analysis [2,4,6-10].
Yokoyama et al. BMC Cancer 2012 12:67 doi:10.1186/1471-2407-12-67