The chimeric antigen receptor F19 is a fusion protein which consists of four functional domains. The scFv F19 serves as binding domain and binds to FAP. The IgG Fc-domain functions as a spacer (hinge); the CD28 signalling domain leads to co-stimulation and the CD3ζ signalling domain activates the T cell if the receptor is cross-linked via antigen binding. The CAR will be expressed in CD8 positive T cells with unknown endogenous specificity.
Petrausch et al. BMC Cancer 2012 12:615 doi:10.1186/1471-2407-12-615