Relative expression of WNT7A in control cells and leukemia-derived cell lines. (A) Relative expression levels of WNT7A determined by qRT-PCR in resting and phytohemagglutinin-activated PBMC (activation was performed in PBMC from two volunteers and named TxPHA 1 and TxPHA 2). Values of the resting PBMCs (Control) of both volunteers were set as 1. (B) Relative expression levels of WNT7A determined by qRT-PCR in Jurkat, CEM, K562, HL60, and BJAB cell lines normalized to the healthy volunteers control group (setting as 1). In A, quantification was calculated using, Ribosomal Protein L32 (RPL32) and Ribosomal Protein S18 (RPS18) as reference genes. In B, quantification was calculated using additionally Glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The graphics show means obtained with both reference genes and Standard deviations (SD). Experiments were carried out at least twice in duplicate.
Ochoa-Hernández et al. BMC Cancer 2012 12:60 doi:10.1186/1471-2407-12-60