Open Access Research article

Peripheral T-lymphocytes express WNT7A and its restoration in leukemia-derived lymphoblasts inhibits cell proliferation

Alejandra B Ochoa-Hernández12, Moisés Ramos-Solano3, Ivan D Meza-Canales3, Beatriz García-Castro3, Mónica A Rosales-Reynoso4, Judith A Rosales-Aviña3, Esperanza Barrera-Chairez5, Pablo C Ortíz-Lazareno3, Georgina Hernández-Flores3, Alejandro Bravo-Cuellar3, Luis F Jave-Suarez3, Patricio Barros-Núñez1 and Adriana Aguilar-Lemarroy36*

Author Affiliations

1 División de Genética, Centro de Investigación Biomédica de Occidente (CIBO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara, Jalisco, Mexico

2 Programa de Doctorado en Genética Humana, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico

3 División de Inmunología, CIBO-IMSS, Guadalajara, Jalisco, Mexico

4 División de Medicina Molecular, CIBO-IMSS, Guadalajara, Jalisco, Mexico

5 Servicio de Hematología, Hospital Civil de Guadalajara Fray Antonio Alcalde, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico

6 División de Inmunología, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada No. 800, Col. Independencia, 44340 Guadalajara, Jalisco, Mexico

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BMC Cancer 2012, 12:60  doi:10.1186/1471-2407-12-60

Published: 7 February 2012

Additional files

Additional file 1:

Relative expression analysis comparison among five of the most used reference genes. A panel of five different reference genes was used to calculate WNT7A expression. Amplification curves of all genes are shown in BJAB cells treated or not with Doxycycline (+Doxy). Standard curves were performed to calculate error and efficiency in all genes used. Table show WNT7A relative expression normalized with the different reference genes.

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Additional file 2:

Amplification curves obtained in cells treated or not with PHA. Upper panel graphics shown the Amplification curves obtained for WNT7A in non-treated PBMC and in PBMC treated for 48 h with PHA. Middle graphics correspond to RPS18 and bottom panel graphics to RPL32. Melting peaks are also shown in the right panels, which indicate the specificity of the PCR reaction.

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Open Data