Additional file 1.
Parental MDA-MB-231, BR3, BR and shRNA transfected variant cells were seeded in 96-well plates (100 μl/well) at a concentration of 1×106 cells/ml and cultured at 37ºC, 5% CO2 in MEM medium. For time-dependent assays, cells were incubated for 24 h, 48 h and 72 h. Cell viability was analyzed using MTT (3 (4, 5-dimethylthiazol-2yl)-2, 5-diphenyltetrazolium bromide). Statistical comparison by Student’s t-test is expressed as p > 0.05 for both BR and BR3 groups.
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Liu et al. BMC Cancer 2012 12:583 doi:10.1186/1471-2407-12-583