Table 1 |
||||
| Immunostaining protocol details listed by antigen | ||||
| CD31 | Collagen IV | αSMA | LYVE-1 | |
| NGS blocking incubation time | 1 h | 1 h | 1 h | 20 min |
| Primary antibody dilution | 1:200 | 1:1000 | 1:1000 | 1:1000 |
| Secondary antibody incubation time | 1 h | 30 min | 30 min | 30 min |
| ABC reagent incubation time | 1 h | 30 min | 30 min | 30 min |
Ho et al. BMC Cancer 2012 12:579 doi:10.1186/1471-2407-12-579
