Additional file 4.
Figure S1. Melt curve analyses of bisulfite-modified and amplified PLA2R1 gene sequences in bone marrow aspirates of MDS patients. After isolation and subsequent bisulfite modification of genomic DNA from bone marrow aspirates melt properties of amplified PLA2R1 sequences covering 5`-CpG sites 6–14 (−437 bp to −270 bp from exon 1) were analyzed using RotorGene Q. P34; a 74 years old male patient with RCMD and low-risk according to IPSS classification, P57; a 69 years old male patient with RAEB-2 and intermediate-1-risk, P66; a 69 years old female patient with RAEB-2 and intermediate-2-risk, and P69; a 56 years old male patient with AML and high-risk classification. Individual data of MDS patients are summarized in Additional file 2: Table S2. Melt curves of amplified genomic DNA from MDS patients (in colors) and those of unmethylated (0%) and methylated (100%) standard DNA samples (in black) are shown. Fluorescence dF/dT were measured. Analyses were performed in duplicates and results are representative of three independent measurements.
Format: DOCX Size: 17KB Download file
Menschikowski et al. BMC Cancer 2012 12:576 doi:10.1186/1471-2407-12-576