Open Access Research article

LINE-1 methylation shows little intra-patient heterogeneity in primary and synchronous metastatic colorectal cancer

Aika Matsunoki12, Kazuyuki Kawakami1*, Masanori Kotake12, Mami Kaneko12, Hirotaka Kitamura12, Akishi Ooi3, Go Watanabe2 and Toshinari Minamoto1

Author Affiliations

1 Division of Translational and Clinical Oncology, Cancer Research Institute, Kanazawa University, 13-1 Takara-machi, Kanazawa, 920-0934, Japan

2 Department of General and Cardiothoracic Surgery, Kanazawa University Graduate School of Medical Science, 13-1 Takara-machi, Kanazawa, 920-8641, Japan

3 Department of Molecular and Cellular Pathology, Kanazawa University Graduate School of Medical Science, 13-1 Takara-machi, Kanazawa, 920-8641, Japan

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BMC Cancer 2012, 12:574  doi:10.1186/1471-2407-12-574

Published: 5 December 2012

Additional files

Additional file 1:

Figure S1. Representative images of tissue sections taken before and after LCM.

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Additional file 2:

Figure S2. Methods for the synthesis of assay standards.

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Additional file 3:

Figure S3. Accuracy of newly developed LINE-1 MethyLight assay.

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Additional file 4:

Table S1. Results of four independent assays for LINE-1 methylation.

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Additional file 5:

Table S2. LINE-1 methylation levels in primary tumor of CRC patients. Samples are obtained from tumor center, invasive front and other area.

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Additional file 6:

Figure S4. Relationship between the LINE-1 methylation level measured by MethyLight assay and those measured by qMSP assay.

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