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Open Access Case report

Pyloric gland adenoma of the cystic duct with malignant transformation: report of a case with a review of the literature

Inga-Marie Schaefer1*, Silke Cameron4, Peter Middel4, Kia Homayounfar3, Harald Schwörer2, Michael Vieth4 and Lothar Veits4

Author Affiliations

1 Department of Pathology, University Medical Center Göttingen, Robert-Koch-Straße 40, Göttingen, D-37075, Germany

2 Gastroenterology and Endocrinology, University Medical Center Göttingen, Göttingen, Germany

3 General and Visceral Surgery, University Medical Center Göttingen, Göttingen, Germany

4 Institute of Pathology, Klinikum Bayreuth, Germany

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BMC Cancer 2012, 12:570  doi:10.1186/1471-2407-12-570


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1471-2407/12/570


Received:22 September 2012
Accepted:29 November 2012
Published:4 December 2012

© 2012 Schaefer et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Pyloric gland adenoma consists of closely packed pyloric-type glands lined by mucus-secreting cells. To date, approximately 230 cases have been reported, mostly of gastric localization with a tumour size up to 3.5 cm and a mean age of occurrence around 70 years. Adenocarcinoma develops in about 40% of cases and may be difficult to detect due to relatively mild nuclear atypia.

Case presentation

We present the first case of a pyloric gland adenoma of the cystic duct in a 62-year-old male patient and demonstrate the clinicopathologic characteristics, including radiographic, molecular, and cytogenetic findings. The 2 cm-tumour developed in the cystic duct and protruded into the hepatic and common bile duct. On microscopic examination, it displayed closely packed pyloric-type glands, and focal architectural distortion with mild nuclear atypia. Immunohistochemically, it expressed MUC1, MUC5AC, MUC6 and p53, but not MUC2 and CD10. The Ki67-proliferation index was 25%. Furthermore, high-grade intraepithelial neoplasia was observed in the surrounding bile duct. We detected chromosomal gains at 7p, 7q11q21, 15q, 16p, 20, losses at 6p23pter, 6q, 18, and amplifications at 1q and 6p21p22 in the pyloric gland adenoma by comparative genomic hybridization. A KRAS codon 12 mutation (c.35G>T; p.G12V) was detected in the pyloric gland adenoma and in the adjacent dysplasia by sequencing analysis. The diagnosis of pyloric gland adenoma was established with transition into well-differentiated adenocarcinoma and high-grade biliary intraepithelial neoplasia.

Conclusion

Pyloric gland adenoma evolving in the cystic duct is a rare differential diagnosis of obstructive bile duct tumours. Other premalignant bile duct lesions may be associated. Due to the risk of developing adenocarcinoma, surgical resection should be performed.

Keywords:
Pyloric gland adenoma; Adenocarcinoma; Cystic duct; Comparative genomic hybridization (CGH); KRAS mutation

Background

Pyloric gland adenoma was first described in 1976 by Kurt Elster. At that time, a neoplasm was not recognized, but since 1990 pyloric gland adenoma has been categorized as a distinct neoplastic entity in the WHO classification of gastric tumours [1-3]. In the approximately 230 previously reported cases, the lesion was mostly localized in the stomach (69%), followed by gallbladder (14%), duodenum (12%), esophagus, gastroesophageal junction, bile duct, pancreatic duct, and rectum (together <5%) [2-15]. In the stomach, the pyloric gland adenoma accounts for <3% of gastric polyps [3]. Extra-gastric cases are even rarer and their incidence is not known [3]. However, pyloric gland adenoma is reported to be the most common type of benign epithelial neoplasm of the gallbladder, although it rarely occurs in the extrahepatic bile ducts [16]. The lesion occurs in patients with a mean age of approximately 70 years, with a reported mean tumour size of 0.6-3.5 cm, and a slight female predominance [2-15]. It harbors the risk of malignant transition into adenocarcinoma, occurring in up to 47% of cases of all locations [3]. The diagnosis of pyloric gland adenoma can be established according to the histological criteria proposed by Watanabe et al.: closely packed pyloric-type glands, lined by cuboidal or columnar mucus-secreting cells with round or oval, relatively small, hyperchromatic nuclei with a parabasal location; so-called lateral expansion or fusion of neighboring foveolae indicate adenocarcinoma [3].

Three cases of pyloric gland adenoma of the common bile duct have up to now been reported [7]. Here, we present the first reported case of pyloric gland adenoma evolving in the cystic duct, with transition into well-differentiated adenocarcinoma, and associated high-grade intraepithelial neoplasia of the adjacent bile duct. The clinico-pathologic characteristics, including radiologic as well as molecular and cytogenetic findings, will be demonstrated with a review of the literature.

Case presentation

A 62-year-old male patient was admitted with a three-week history of colic-like pain in the upper abdomen and jaundice. He had a metabolic syndrome (body mass index 45 kg/m2) including a fatty liver disease with beginning fibrosis, and a history of smoking (25 pack years). Abdominal computed tomography (CT) revealed an approximately 3 × 2 cm polypoid mass lesion apparently located in the common bile duct and along the bifurcation into the cystic duct with consecutive dilation of the central intra- and extrahepatic bile ducts (Figure 1A, B), gallbladder hydrops and cholecystolithiasis. Laboratory tests detected an increase in total bilirubin (1.4 mg/dL; normal ≤1.2 mg/dL), aspartate amino transferase (76 U/L; normal ≤35 U/L), alanine amino transferase (79 U/L; normal ≤45 U/L), and γ-glutamyl transferase (223 U/L; normal ≤55 U/L). Levels of alpha-fetoprotein (AFP) and carbohydrate antigen 19–9 (CA19-9) were within normal range (AFP 3 μg/L; normal <7 μg/L, and CA19-9 31 kU/L; normal <37 kU/L). As abdominal ultrasound showed a dilated common hepatic duct of up to 2 cm, endoscopic retrograde cholangiopancreatography (ERCP) was performed (Figure 1C). It revealed a mass in the common hepatic duct and hematobilia. Via passage with a blocked balloon, material was obtained for histoxpathology. A stent was inserted into the bile duct, producing immediate bile drainage. After that intervention, the jaundice steadily declined and the cholestatic parameters normalized. The initial pathological diagnosis of the obtained tissue was a tubulo-villous adenoma of the bile duct.

thumbnailFigure 1. Radiographic findings of the pyloric gland adenoma of the cystic duct. Abdominal computed tomography revealed markedly dilated intrahepatic bile ducts (A, arrow), and a polypoid tumour in the common hepatic duct just below the bifurcation (B, arrow). Endoscopic retrograde cholangiopancreatography confirmed the polypoid mass lesion (C, arrow) and demonstrated consecutive dilation of the central intrahepatic bile ducts. Endosonography verified the polypoid intraluminal tumour of 2.1 x 1.1 cm in the common hepatic duct (D, arrow) next to the portal vein.

After a four-week interval for weight reduction, ERCP was re-performed and the stent was removed. Fluoroscopic guidance with contrast application revealed the tumour in the middle part of the common bile duct. After obtaining biopsies from the tumour, a stent was inserted again for drainage until the operation. Endosonography located the tumour in the common bile duct (Figure 1D) and the cystic duct, protruding into the infundibulum of the gallbladder. A suspect lymph node was detected between bile duct and cystic duct.

Histopathological examination revealed tubulo-papillary neoplastic proliferations and closely packed glandular structures with eosinophilic cytoplasm, round to oval nuclei and inconspicuous nucleoli. At the surface, small papillary proliferations were observed. Focally, marked architectural distortion with nuclear atypia, hyperchromatic nuclei with prominent nucleoli, and a back-to-back formation of stellar glands were present. Squamous morules were not observed. The diagnosis of a pyloric gland adenoma with possible transition into well-differentiated adenocarcinoma was established and confirmed by reference pathology.

In the meantime the patient developed a thrombosis of the right cephalic vein after catheter infection and a non-ST-elevation myocardial infarction, which were treated non-interventionally with antibiotics and heparin for prolongation of the prothrombin time. Two weeks later, resection of the extrahepatic bile ducts including gallbladder with biliodigestive anastomosis was performed.

The resected specimen of the common bile duct, cystic duct with attached gallbladder, and common hepatic duct presented a 2.5 × 2 cm tumour with a gray-brown cut surface, developing in the cystic duct, and protruding through the bifurcation into both the common bile duct and common hepatic duct, with partial obstruction of the lumen (Figure 2). The surrounding cystic duct showed epithelial cell proliferations in the mucosa with moderate and focal high-grade cellular atypia, outstretching into small branching bile duct (Figure 3). Immunohistochemical staining with MUC1 (clone MRQ-17, 1:300, Cell marque/Medac, Wedel, Germany), MUC2 (clone MRQ-18, 1:100, Cell marque/Medac), MUC5AC (clone MRQ-19, 1:300, Cell marque/Medac), MUC6 (clone MRQ-20, 1:300, Cell marque/Medac), vascular endothelial growth factor receptor (VEGF; clone SP28, prediluted, Abcam, Cambridge, MA, USA), CD10 (clone 56C6, 1:25, Zytomed Systems, Berlin, Germany), CDX2 (clone EPR2764Y, 1:100, Cell Marque, Rocklin, CA, USA), p53 (clone DO-7, 1:50, Dako, Glostrup, Denmark), p21 (clone DCS-60.2, 1:100, Thermo Scientific, Fremont, CA, USA), p16 (clone JC8, 1:100, Santa Cruz Biotechnology, Heidelberg, Germany), and Ki67 (clone K-2, 1:200 Zytomed Systems) was performed. The pyloric gland adenoma showed focal positive staining for MUC1, and negative staining for MUC2, as well as superficial staining for MUC5AC, and positive expression of MUC6 and VEGF (Figure 4). CD10 and CDX2 were not expressed. Nuclear expression of p53, focal p16, and p21 was observed. Proliferative activity was assessed by Ki67 and estimated at 25%. The high-grade intraepithelial neoplasia of the bile duct, in contrast, did not express MUC1, MUC2, and MUC5AC, but MUC6 and VEGF. Staining with CD10 and CDX2, p53, and p16 was negative, whereas p21 was only focally expressed. Proliferative activity was assessed by Ki67, and estimated at 10%. The diagnosis of a pyloric gland adenoma with focal high-grade intraepithelial neoplasia and transition into well-differentiated gastric-type adenocarcinoma associated with BilIN-3 of the cystic duct resembling gastric-type intraductal papillary neoplasm in areas with low-grade intraepithelial neoplasia was thus confirmed histopathologically, and the tumour was finally staged at pT1, pNX, pMX, G1, L0, V0, R0. Metastases had been excluded by CT scan.

thumbnailFigure 2. Gross findings of the resected pyloric gland adenoma. The resected specimen comprised the common bile duct (star), the bifurcation into common hepatic duct (arrow) and cystic duct with attached gallbladder (A). Cross sections revealed an intraluminal tumour of 2.5 cm length and 2 cm in diameter developing in the cystic duct (B), and protruding into the common bile duct and common hepatic duct (C, arrow).

thumbnailFigure 3. Microscopic findings of the pyloric gland adenoma and adjacent bile duct lesions. On microscopic view, the pyloric gland adenoma arose in the cystic duct (right) and displayed a papillary, intraluminal growth pattern with protrusion into the common bile duct (left) (A, H&E, x40). Closely packed pyloric type glands were lined by cuboidal to columnar mucus-secreting cells (B, x100) with focal architectural distortion (C, x100) and high-grade dysplasia with nuclear atypia, indicating transition into well-differentiated adenocarcinoma (D, x200). Focal high-grade intraepithelial neoplasia (BilIN-3) of the cystic duct was detected (E, x200), focally resembling gastric-type intraductal papillary neoplasm (IPN) with direct transition into the pyloric gland adenoma (F, x200).

thumbnailFigure 4. Immunohistochemical findings of the pyloric gland adenoma and adjacent bile duct lesions. The pyloric gland adenoma (A, HE) immunohistochemically expressed focal MUC1 (B), but no MUC2 (C). MUC5AC (D) was positive predominantly in the superficial luminal cell layers, whereas MUC6 (E) was expressed throughout. The tumour cell also expressed vascular endothelial growth factor receptor (VEGF) (F), but no CD10 (G) and CDX2 (H). Nuclear p53 (I) was positive, p16 (J) was focally observed, and p21 (K) was positive. Ki67 (L) was observed in approximately 25% (x100).

CGH analysis was performed from the pyloric gland adenoma as described previously [17] and revealed chromosomal gains at 7p, 7q11q21, 15q, 16p, 20, losses at 6p23pter, 6q, 18, and amplifications at 1q and 6p21p22 in the pyloric gland adenoma (Figure 5). Sequencing analysis of KRAS exon 1 and 2 showed a point mutation at exon 1, codon 12 (c.35G>T; p.G12V) and wildtype sequence at exon 2 in the pyloric gland adenoma and the same mutation in the adjacent BilIN-3. The patient recovered well and was discharged 12 days after surgery. He presently shows no signs of tumour relapse 12 months after tumour resection.

thumbnailFigure 5. Results of comparative genomic hybridization (CGH). CGH of the pyloric gland adenoma revealed ish cgh amp(1)(q),amp(6)(p21p22),dim(6)(p23pter),dim(6)(q),enh(7)(p),enh(7)(q11q21),enh(15)(q),enh(16)(p),dim(18),enh(20) as indicated by green (gains) and red (losses) bars. The number of chromosomes included in the CGH analysis is indicated at the bottom of each individual profile.

The clinico-pathologic findings of previously reported cases of pyloric gland adenoma [2-15] as well as the present case are summarized in Table 1. The developmental etiology of pyloric gland adenoma is still unclear, particularly when observed in localizations other than gastric. However, the sequence of metaplasia-dysplasia and carcinoma is well accepted as a histogenetic pathway of the gallbladder and bile duct carcinogenesis [16,18]. For the development of pyloric gland adenoma/adenocarcinoma, it is believed that the first step may be initiated by the presence of gastric metaplasia or gastric heterotopias [6,7]. An association of pyloric gland adenomas with heterotopic gastric mucosa or gastric metaplasia in the gallbladder [14], pancreas [4], duodenum [15], and rectum [6] has previously been reported. Above the designation as hyperplasia or hamartoma, some authors suggest an unstable and precancerous nature by proposing a “pyloric gland adenoma-adenocarcinoma sequence” by CGH analyses, and underline its high potential for invasive malignancy [3,18]. Immunohistochemically, as in the present case, the tumour typically expresses MUC6 and variably MUC5AC [2,3,6,8,9,11,12,14,15]. MUC2 and CD10 are generally negative, but may indicate transition from gastric to intestinal differentiation [2,3,8,12]. Additionally, Ki67 expression and p53 mutations can be used to detect malignant transformation [3]. The presence of adenocarcinoma may be difficult to recognize since the cytology of adenocarcinoma developing in pyloric gland adenoma is known to show rather mild abnormalities without the classical signs of intraepithelial neoplasia/dysplasia [3]. However, transition from round or oval nuclei to elongated or pleomorphic nuclei with loss of polarity in conjunction with so-called lateral expansion or branching of glands indicates the presence of adenocarcinoma, as found focally also in the present case (Figure 3) [3]. In another study describing 3 pyloric gland adenocarcinomas of the extrahepatic bile ducts the authors observed pyloric gland metaplasia adjacent to the adenocarcinoma in 2 of 3 cases and suggested them as probable precursor lesion [16]. These tumours did not arise from pyloric gland adenomas, but displayed a similar immunophenotype with positive expression of MUC5AC and MUC6 and negativity for MUC2 and CDX2 [16]. Squamous morules (or spindle cell metaplasias) are reported to occur in 23% of cases [9], but were not observed in the present case.

Table 1. The clinico pathologic characteristics of previously reported cases of pyloric gland adenoma [2-15] and the case reported here

Histopathological differential diagnoses of polypous intraductal bile duct lesions include adenomas of the gallbladder and extrahepatic bile ducts. They can be divided into a tubular, papillary, and tubulopapillary type based on their growth pattern, and cytologically into a pyloric-gland, intestinal, foveolar, and biliary type [1]. IPN of gastric, pancreatobiliary, intestinal or oncocytic phenotype, and mucinous cystic neoplasms should also be considered [1]. Furthermore, concomitant intraductal papillary mucinous neoplasms (IPMN) [5,13] have been observed in cases of pancreatic pyloric gland adenomas. In the pancreas, gastric-type IPMN are usually located as small cystic lesions in branching ducts, harboring only mild/low-grade atypia and immunohistochemically expressing MUC5AC, but not MUC1, MUC2 or CDX2. They are associated with a rather favorable clinical prognosis compared to the other subtypes of IPMN [19]; adenocarcinoma occurs in 10-15% [19]. Invasive adenocarcinoma may also develop in IPN of the bile duct [1]. BilIN of the gallbladder and extrahepatic bile ducts are associated with lithiasis in up to 3%, familial adenomatous polyposis coli, sclerosing cholangitis, and pancreatobiliary reflux [1]. Additionally, BilIN-3 usually arises in a association with pyloric and intestinal metaplasia, as observed in the present case, with an abrupt transition between normal and atypical columnar cells [1]. As reported, BilIN immunohistochemically also expresses p53 [1].

KRAS codon 12 mutations have been previously reported in two cases of pyloric gland adenoma of the main pancreatic duct, and probably support the neoplastic nature of this tumour [4,5]. KRAS (13-100%) and TP53 (50%) mutations have been described for IPMN of the pancreas before [19]. Also in the present case, the pyloric gland adenoma and the BilIN-3 harbored a KRAS codon 12 mutation, indicating a possible metaplasia-dysplasia-carcinoma sequence with a common tumourigenesis.

Previous CGH results of a pyloric gland adenoma of the esophagus revealed chromosomal aberrations which overlapped with findings in Barrett’s dysplasia and adenocarcinoma as well as gastric cardia adenocarcinoma [8]. In pyloric gland adenomas of the stomach, previous CGH analyses revealed chromosomal abnormalities common to invasive gastric adenocarcinoma, including -5q (50%), -6 (40%), -4q, +17pq and +20 [18,20]. Additional gains were observed at 1, 3q, 5q, 7, 9q, 11q, 12q, 13q, 15q, 17 and 22q, and losses at 1p, 2q, 4, 9p, 10, 12q 13q, 14q, 16, 18q, 20q, and 21 [20]. Of these aberrations, gains at 7p and 15q, and losses at 6q, and 18q were also detected in the present case. Interestingly, losses at 6q and 18q have been demonstrated in pancreatic IPMN, before [21]. Furthermore, the amplicon at 6p21p22 harbors the VEGF (VEGF-A) gene at 6p21.1 (MIM ID *192240) which was shown to be expressed by the pyloric gland adenoma by immunohistochemical staining, suggesting VEGF upregulation. VEGF plays a crucial role in angiogenesis of normal tissues and several types of tumours [22]. Altogether, the relatively high number of chromosomal imbalances in the present case of pyloric gland adenoma of the cystic duct suggests an instable karyotype and underlines the risk of malignant transformation.

Conclusions

In conclusion, a pyloric gland adenoma evolving in the cystic duct is very rare, but may sometimes be overlooked and therefore should be considered as a differential diagnosis for obstructive bile duct tumours. An association with other premalignant bile duct lesions such as BilIN may be observed. ERCP-guided biopsy with histopathological examination is necessary to establish the diagnosis. Due to the high risk of evolving adenocarcinoma, surgical resection should be performed whenever possible.

Consent

Written informed consent was obtained from the patient for publication of this Case report and any accompanying images. A copy of the written consent is available for review by the Series Editor of this journal.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

IMS, PM, LV, and MV performed the histopathological, immunohistochemical and genetic examinations and established the diagnosis. SC, KH, and HS examined, treated and observed the patient, including follow-up. IMS, SC, PM, KH, HS, LV, and MV participated in writing the manuscript. SC and HS provided the radiographic, and IMS the histological and CGH images. All authors read and approved of the final manuscript.

Acknowledgements

The authors thank Sabine Schäfer, Radiology group practice Göttingen, Germany, for providing radiographic images.

References

  1. Bosman FT, Carneiro F, Hruban RH, Theise ND: WHO classification of tumours of the digestive system. Lyon: International Agency for Research on Cancer; 2010. OpenURL

  2. Chen ZM, Scudiere JR, Abraham SC, Montgomery E: Pyloric gland adenoma: an entity distinct from gastric foveolar type adenoma.

    Am J Surg Pathol 2009, 33:186-193. PubMed Abstract | Publisher Full Text OpenURL

  3. Vieth M, Kushima R, Mukaisho K, Sakai R, Kasami T, Hattori T: Immunohistochemical analysis of pyloric gland adenomas using a series of Mucin 2, Mucin 5AC, Mucin 6, CD10, Ki67 and p53.

    Virchows Arch 2010, 457:529-536. PubMed Abstract | Publisher Full Text OpenURL

  4. Bakotic BW, Robinson MJ, Sturm PD, Hruban RH, Offerhaus GJ, Albores-Saavedra J: Pyloric gland adenoma of the main pancreatic duct.

    Am J Surg Pathol 1999, 23:227-231. PubMed Abstract | Publisher Full Text OpenURL

  5. Kato N, Akiyama S, Motoyama T: Pyloric gland-type tubular adenoma superimposed on intraductal papillary mucinous tumor of the pancreas.

    Pyloric gland adenoma of the pancreas. Virchows Arch 2002, 440:205-208. OpenURL

  6. Vieth M, Kushima R, de Jonge JP, Borchard F, Oellig F, Stolte M: Adenoma with gastric differentiation (so-called pyloric gland adenoma) in a heterotopic gastric corpus mucosa in the rectum.

    Virchows Arch 2005, 446:542-545. PubMed Abstract | Publisher Full Text OpenURL

  7. Vieth M, Kushima R, Borchard F, Stolte M: Pyloric gland adenoma: a clinico-pathological analysis of 90 cases.

    Virchows Arch 2003, 442:317-321. PubMed Abstract | Publisher Full Text OpenURL

  8. Kushima R, Vieth M, Mukaisho K, Sakai R, Okabe H, Hattori T, Neuhaus H, Borchard F, Stolte M: Pyloric gland adenoma arising in Barrett's esophagus with mucin immunohistochemical and molecular cytogenetic evaluation.

    Virchows Arch 2005, 446:537-541. PubMed Abstract | Publisher Full Text OpenURL

  9. Wani Y, Notohara K, Fujisawa M: Aberrant expression of an "intestinal marker" Cdx2 in pyloric gland adenoma of the gallbladder.

    Virchows Arch 2008, 453:521-527. PubMed Abstract | Publisher Full Text OpenURL

  10. Golger D, Probst A, Wagner T, Messmann H: Pyloric-gland adenoma of the stomach: case report of a rare tumor successfully treated with endoscopic submucosal dissection.

    Endoscopy 2008, 40(Suppl 2):E110-E111. PubMed Abstract | Publisher Full Text OpenURL

  11. Oh MG, Cho SJ, Lee JH, Kook MC, Park SY: A spongiform mass in the stomach: pyloric gland adenoma with a transition to adenocarcinoma.

    Korean J Gastroenterol 2010, 56:1-5. PubMed Abstract | Publisher Full Text OpenURL

  12. Gutierrez-Grobe Y, Gavilanes-Espinar J, Uribe M, Kobashi-Margain R, Mendez-Sanchez N: Pyloric Gland Adenoma: Case Report.

    Rev Gastroenterol Mex 2010, 75:360-362. PubMed Abstract OpenURL

  13. Amaris J: Intraductal mucinous papillary tumor and pyloric gland adenoma of the pancreas.

    Gastrointest Endosc 2002, 56:441-444. PubMed Abstract | Publisher Full Text OpenURL

  14. Kushima R, Remmele W, Stolte M, Borchard F: Pyloric gland type adenoma of the gallbladder with squamoid spindle cell metaplasia.

    Pathol Res Pract 1996, 192:963-969. PubMed Abstract | Publisher Full Text OpenURL

  15. Kushima R, Ruthlein HJ, Stolte M, Bamba M, Hattori T, Borchard F: 'Pyloric gland-type adenoma' arising in heterotopic gastric mucosa of the duodenum, with dysplastic progression of the gastric type.

    Virchows Arch 1999, 435:452-457. PubMed Abstract | Publisher Full Text OpenURL

  16. Albores-Saavedra J, Chable-Montero F, Mendez-Sanchez N, Mercado MA, Vilatoba-Chapa M, Henson DE: Adenocarcinoma with pyloric gland phenotype of the extrahepatic bile ducts: a previously unrecognized and distinctive morphologic variant of extrahepatic bile duct carcinoma.

    Hum Pathol 2012, 43:2292-8. PubMed Abstract | Publisher Full Text OpenURL

  17. Schaefer IM, Martinez R, Enders C, Loertzer H, Bruck W, Rohde V, Fuzesi L, Gutenberg A: Molecular cytogenetics of malignant pheochromocytoma with cerebral metastasis.

    Cancer Genet Cytogenet 2010, 200:194-197. PubMed Abstract | Publisher Full Text OpenURL

  18. Kushima R, Vieth M, Borchard F, Stolte M, Mukaisho K, Hattori T: Gastric-type well-differentiated adenocarcinoma and pyloric gland adenoma of the stomach.

    Gastric Cancer 2006, 9:177-184. PubMed Abstract | Publisher Full Text OpenURL

  19. Sipos B, Henopp T: Precursor lesions of pancreatobiliary cancer.

    Pathologe 2011, 32:224-31. PubMed Abstract | Publisher Full Text OpenURL

  20. Buffart TE, Carvalho B, Mons T, Reis RM, Moutinho C, Silva P, van Grieken NC, Vieth M, Stolte M, van de Velde CJ, et al.: DNA copy number profiles of gastric cancer precursor lesions.

    BMC Genomics 2007, 8:345. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  21. Fritz S, Fernandez-del CC, Mino-Kenudson M, Crippa S, Deshpande V, Lauwers GY, Warshaw AL, Thayer SP, Iafrate AJ: Global genomic analysis of intraductal papillary mucinous neoplasms of the pancreas reveals significant molecular differences compared to ductal adenocarcinoma.

    Ann Surg 2009, 249:440-447. PubMed Abstract | Publisher Full Text OpenURL

  22. Ferrara N: Vascular endothelial growth factor: basic science and clinical progress.

    Endocr Rev 2004, 25:581-611. PubMed Abstract | Publisher Full Text OpenURL

Pre-publication history

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