Salinomycin induces phosphorylation of Akt. Western blotting shows an increase in phosphorylation of Akt (Ser473), as well as the immediate downstream targets GSK3-β (Ser9) and mTOR (Ser2448), when treated with the indicated doses of salinomycin. Total Erk is utilized as a loading control.
Kuo et al. BMC Cancer 2012 12:556 doi:10.1186/1471-2407-12-556