Standard curves of CADM1 and MAL in a multiplex qMSP with ACTB* tested on a serial dilution series of methylation positive SiHa DNA in methylation negative DNA of primary keratinocytes. Standard curve of CADM1 (A) and MAL (B) with the previously described primers 9. CADM1 (C) and MAL (D) primers with nearly identical annealing temperatures as ACTB primers. For the dilution series the PCR efficiencies were calculated by E=(10(−1/Slope)-1)×100%. The qPCR efficiencies increased from 76% to 87% for CADM1 and 52% to 105% for MAL.*) Multiplex qMSP was performed with an ACTB primer concentration of 200 nM.
Snellenberg et al. BMC Cancer 2012 12:551 doi:10.1186/1471-2407-12-551