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Development of a multiplex methylation-specific PCR as candidate triage test for women with an HPV-positive cervical scrape

Suzanne Snellenberg, Lise MA De Strooper, Albertus T Hesselink, Chris JLM Meijer, Peter JF Snijders, Daniëlle AM Heideman and Renske DM Steenbergen*

Author Affiliations

Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands

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BMC Cancer 2012, 12:551  doi:10.1186/1471-2407-12-551

Published: 23 November 2012

Additional files

Additional file 1:

Reproducibility of multiplex qMSP. Serial dilutions of methylated DNA (SiHa) spiked with unmethylated DNA (EK) in order to obtain a serial dilution of 50 ng to 0.25 ng methylated DNA in a total of 50 ng of DNA, showed high reproducibility when testing the multiplex qMSP in 10-fold.

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