Bacterial expression and purification of Junction region (aa 251- aa 343).A: Coomassie stained SDS PAGE gel picture of Ni-NTA Purification of Junction- EWS-FLI1 protein under native condition. Lane1: Protein ladder, Lane 2: Induced cell lysate soluble fraction, Lane 3: Flow Through, Lane 4: Wash and Lane 5: Elute. Figure 4B: Confirmation of junction region (aa 251–343) protein expression by immunoblotting with anti Thio antibody. Lane 1: insoluble fraction, Lane 2: soluble fraction. Figure 4C: CD of junction region (aa 251-aa 343) of EWS-FLI1 soluble construct.
Jully et al. BMC Cancer 2012 12:513 doi:10.1186/1471-2407-12-513