Bacterial expression and purification of recombinant full length EWS-FLI1.A: Coomassie stained SDS PAGE gel picture showing,expression of Full Length Trx -EWS-FLI1-His tag Protein in insoluble fraction.Lane 1 represents protein ladder, Lane2 represents soluble fraction , Lane 3 represents insoluble fraction. B: Confirmation of EWS-FLI1 protein expression by immunoblotting with anti FLI1 antibody. Lane 1: soluble fraction, Lane 2:insoluble fraction. C: Coomassie stained SDS PAGE gel picture of Ni-NTA Purification of full length EWS-FLI1 protein in denaturing condition.Lane1: Protein ladder Lane 2: Uninduced cell lysate, Lane 3: Induced cell lysate , Lane 4: Flow Through, Lane 5: Wash and Lane 6: Elute.
Jully et al. BMC Cancer 2012 12:513 doi:10.1186/1471-2407-12-513