Table 2

Associations between calpastatin, calpain-1 and calpain-2 protein expression and various clinicopathological variables in the bile duct and ampullary cancer cohort
Variable Calpain-1 Calpain-2 Calpastatin (cytoplasmic) Calpastatin (nuclear)
Low High p value Low High p value Low High p value Low High p value
Age
 ≤ 60 years (n=40) 12 (30.0) 27 (38.6) 0.366 14 (36.8) 22 (34.9) 0.845 16 (50.0) 22 (28.9) 0.036 25 (35.7) 12 (32.4) 0.734
 > 60 years (n=78) 28 (70.0) 43 (61.4) 24 (63.2) 41 (65.1) 16 (50.0) 54 (71.1) 45 (64.3) 25 (67.6)
Tumour size
 ≤ 2cm (n=48) 15 (37.5) 29 (40.8) 0.729 18 (46.2) 21 (33.3) 0.195 17 (51.5) 27 (35.5) 0.118 30 (42.3) 13 (35.1) 0.473
 > 2cm (n=70) 25 (62.5) 42 (59.2) 21 (53.8) 42 (66.7) 16 (48.5) 49 (64.5) 41 (57.7) 24 (64.9)
T stage
 1 (n=3) 0 (0.0) 3 (4.2) 0.262 0 (0.0) 3 (4.7) 0.137 1 (3.0) 2 (2.6) 0.918 0 (0.0) 3 (8.1) 0.026
 2 (n=27) 11 (26.8) 13 (18.3) 7 (17.9) 15 (23.4) 8 (24.2) 17 (22.1) 20 (27.8) 5 (22.9)
 3 (n=86) 28 (68.3) 54 (76.1) 30 (76.9) 46 (71.9) 23 (69.7) 57 (74.0) 50 (69.4) 29 (78.4)
 4 (n=3) 2 (4.9) 1 (1.4) 2 (5.1) 0 (0.0) 1 (3.0) 1 (1.8) 2 (2.8) 0 (0.0)
N stage
 Negative (n=44) 14 (35.9) 28 (41.2) 0.590 14 (37.8) 24 (39.3) 0.882 11 (35.5) 30 (40.5) 0.628 26 (38.2) 15 (41.7) 0.733
 Positive (n=69) 25 (64.1) 40 (58.8) 23 (62.2) 37 (60.7) 20 (64.5) 44 (59.5) 42 (61.8) 21 (58.3)
Vascular invasion
 absent (n=52) 18 (43.9) 30 (42.9) 0.915 18 (47.4) 26 (40.6) 0.506 13 (39.4) 34 (44.7) 0.605 36 (50.0) 11 (29.7) 0.043
 present (n=66) 23 (56.1) 40 (57.1) 20 (52.6) 38 (59.4) 20 (60.6) 42 (55.3) 36 (50.0) 26 (70.3)
Perineural invasion
 absent (n=51) 17 (41.5) 32 (45.1) 0.711 16 (41.0) 30 (46.9) 0.562 15 (45.5) 34 (44.2) 0.900 36 (50.0) 12 (32.4) 0.080
 present (n=68) 24 (58.5) 39 (54.9) 23 (59.0) 34 (53.1) 18 (54.5) 43 (55.8) 36 (50.0) 25 (67.6)
Tumour site
 Proximal bile duct (n=5) 3 (7.3) 1 (1.4) 0.251 1 (2.6) 3 (4.7) 0.418 3 (9.1) 1 (1.3) 0.074 4 (5.6) 0 (0.0) 0.288
 Distal bile duct (n=65) 22 (53.7) 38 (53.5) 24 (61.5) 31 (48.4) 19 (57.6) 39 (50.6) 39 (54.2) 19 (51.4)
 Ampullary (n=50) 16 (39.0) 32 (45.1) 14 (35.9) 30 (46.9) 11 (33.3) 37 (48.1) 29 (40.3) 18 (48.6)

The frequency of observed clinicopathological variables is noted next to the variable subgroup; in some instances data was not available, 2 cases for patient age, 2 cases for tumour size: 1 case for tumour stage, 7 cases for N stage, 2 cases for vascular invasion, and 1 case for perineural invasion. The P values are resultant from Pearson Chi Square test of association (χ2) or Fisher’s Exact test in a 2×2 table if a cell count was less than 5 (indicated by *). Significant P values are indicated by bold font.

Storr et al.

Storr et al. BMC Cancer 2012 12:511   doi:10.1186/1471-2407-12-511

Open Data