Table 3

Antibodies used for immunohistochemical analysis
Antigen Manufacturer Mono-/polyclonal Staining Positive control Blocking Concentration
phospho-Smad1 (Ser463/465) Cell signaling polyclonal rabbit IgG nucleus colon no 1:200
/Smad5 (Ser463/465)
/Smad8 (Ser426/428)
phospho-Smad2 (Ser465/467) Cell signaling monoclonal rabbit IgG1 nucleus kidney 10% normal goat serum, 30’ 1:50
endoglin / CD105 R&D systems polyclonal goat IgG cytoplasmic tonsil no 1:800

Boeuf et al.

Boeuf et al. BMC Cancer 2012 12:488   doi:10.1186/1471-2407-12-488

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