Additional file 1.

Figure S1. Optimization of the annealing temperature of the MS-HRMA assay for CST6 promoter methylation. Normalized melting curves and first derivative plots for a) 63ºC: Black: 0%, red: 1%, blue: 10%, green: 50%, yellow: 100% methylation b) 61ºC: Black: 0%, red: 1%, blue: 10%, yellow: 50%, green: 100% methylation and c) 60ºC: Black: 0%, red: 1%, blue: 10%, green: 50%, yellow: 100% methylation.

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Dimitrakopoulos et al. BMC Cancer 2012 12:486   doi:10.1186/1471-2407-12-486