Unsupervised cluster analysis of phospho-proteins depicts that lymphoma samples can be separated from normal PBMCs. All phospho-proteins with a relative MFI greater or less than 0.25 in the basal or induced conditions were included in the unsupervised cluster analysis. Phospho-proteins and samples were subjected to clustering using complete linkage method. The heatmap illustrates basal and induced MFI phospho-proteins in all SLL/CLL, MZL patients and PBMCs. Basal, unstimulated MFI represent a ratio of MFI in the lymphoma CD20+-subset and MFI in CD20+ normal B cells. Induced signaling are the ratio of MFI in the stimulated CD20+-subset or the CD5+ CD20--subset in case of T cells, and MFI from unstimulated cells in the same subset. Missing data are indicated by grey color.
Blix et al. BMC Cancer 2012 12:478 doi:10.1186/1471-2407-12-478