Table 1

Primer sequences with corresponding annealing temperatures, efficiencies, and product length in basepairs (bp) used for realtime PCR
Gene Primer sequences (sense/antisense) Efficiency Annealing temperature (°C) Product length (bp)
GAPDH 5′;-TGGTATCGTGGAAGGACTCA-3′; 1.93 67 132
5′;-CCAGTAGAGGCAGGGATGAT-3′;
DEFA1/3 5′;-ATGAGGACCCTCGCCATCCTTGCT-3′; 2.17 69 285
5′;-TCAGCAGCAGAATGCCCAGCGTCTTCCC-3′;
DEFA4 5′;-GTCTGCTCTTGCAGATTAGTATTCTG-3′; 1.98 69 105
5′;-TTAATCGACACGCGTGCAGCAGTAT-3′;

PCR efficiencies for every set of primers were determined with dilution series of primer specific cloned PCR-products at the corresponding annealing temperature. Efficiency stands for the performance to amplify a cDNA template. Efficiency values of 2.0 means an amplification of 100%.

Winter et al.

Winter et al. BMC Cancer 2012 12:465   doi:10.1186/1471-2407-12-465

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