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Activation of AKT by hypoxia: a potential target for hypoxic tumors of the head and neck

Hanneke Stegeman1*, Johannes H Kaanders1, Deric L Wheeler2, Albert J van der Kogel1, Marieke M Verheijen1, Stijn J Waaijer1, Mari Iida2, Reidar Grénman3, Paul N Span1 and Johan Bussink1

Author Affiliations

1 Department of Radiation Oncology, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB, Nijmegen, The Netherlands

2 Department of Human Oncology, University of Wisconsin School of Medicine and Public Health, 1111 Highland Ave, Madison, WI 53705, USA

3 Department of Otorhinolaryngology-Head and Neck Surgery and Department of Medical Biochemistry, Turku University Hospital and University of Turku, PO Box 52, FI-20521, Turku, Finland

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BMC Cancer 2012, 12:463  doi:10.1186/1471-2407-12-463

Published: 10 October 2012

Additional files

Additional file 1:

Figure S1.In vitro and in vivo expression of (p)HER2, (p)STAT3 and (p)ERK1/2 in 6 HNSCC lines. Cell lines were both cultured as cell lines (in vitro) and grown as xenograft tumors (in vivo) and expression levels were determined with western blot. A) In vitro expression of (p)HER2, (p)STAT2 and (p)ERK1/2. B) In vivo expression of (p)HER2, (p)STAT2 and (p)ERK1/2. Number of harvested tumors ranged from 2 to 4 per cell line.

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Additional file 2:

Figure S2. Enlarged detail of Figure 3. Expression of EGFR, pAKT and hypoxia in a tumor of UT-SCC5. Left picture: EGFR (red), pAKT (green), vessels (blue). Right picture: EGFR (red), hypoxia (green), vessels (blue). Magnification: 100X.

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