Table 4

Verification of overall variability and variability of the EP scores, Cqvalues, and normalized ΔCqvalues at the molecular-pathological laboratory at the Charité
Standard deviations
Cq ΔCq EP
variables
 day 0.034 0.032 0.093
 day time 0.062 0.073 0.074
 sample position <0.001 0.016 <0.001
 reagent lot 0.182 0.031 <0.001
 operator 0.035 0.073 0.041
total variable noise 0.198 0.114 0.125
replicate noise 0.131 0.085 0.126
total noise 0.238 0.142 0.178

Different operating conditions (variable noise, replicate noise and total noise) are indicated as standard deviations of the Cq values, ΔCq values, and the EP scores.

Kronenwett et al.

Kronenwett et al. BMC Cancer 2012 12:456   doi:10.1186/1471-2407-12-456

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