Table 3

Verification of linear dynamic range and PCR efficiency at the molecular-pathological laboratory at the Charité
Gene Linear range [log2dilution step] Linear range [Cqvalue] Efficiency [%]
AZGP1 −7 to 0 28.5 to 20.5 82.7 (79.1 - 86.7)
CALM2 −7 to 0 28.7 to 21.5 97.6 (95.7 - 99.7)
BIRC5 −7 to 0 34.2 to 26.6 89.7 (86.4 - 93.2)
DHCR7 −7 to 0 32.4 to 24.4 83.2 (79.2 - 87.7)
IL6ST −7 to 0 31.7 to 23.3 78.0 (74.4 - 81.9)
MGP −7 to 0 28.9 to 20.0 72.3 (68.1 - 77.2)
OAZ1 −7 to 0 30.4 to 22.6 86.7 (83.6 - 90.1)
RBBP8 −7 to 0 33.5 to 25.8 87.6 (84.4 - 91.0)
STC2 −7 to 0 32.1 to 23.9 81.2 (77.7 - 85.0)
UBE2C −7 to 0 32.7 to 24.3 78.1 (74.4 - 82.2)
RPL37A −7 to 0 26.6 to 18.7 85.3 (83.1 - 87.5)
HBBV2 −6 to 0 32.7 to 25.6 79.0 (74.7 - 83.9)

95% confidence intervals are indicated in brackets. For linear range a maximum deviation from linearity of 1 Ct value was accepted.

Kronenwett et al.

Kronenwett et al. BMC Cancer 2012 12:456   doi:10.1186/1471-2407-12-456

Open Data