Table 3 |
|||
| Verification of linear dynamic range and PCR efficiency at the molecular-pathological laboratory at the Charité | |||
| Gene | Linear range [log2dilution step] | Linear range [Cqvalue] | Efficiency [%] |
| AZGP1 | −7 to 0 | 28.5 to 20.5 | 82.7 (79.1 - 86.7) |
| CALM2 | −7 to 0 | 28.7 to 21.5 | 97.6 (95.7 - 99.7) |
| BIRC5 | −7 to 0 | 34.2 to 26.6 | 89.7 (86.4 - 93.2) |
| DHCR7 | −7 to 0 | 32.4 to 24.4 | 83.2 (79.2 - 87.7) |
| IL6ST | −7 to 0 | 31.7 to 23.3 | 78.0 (74.4 - 81.9) |
| MGP | −7 to 0 | 28.9 to 20.0 | 72.3 (68.1 - 77.2) |
| OAZ1 | −7 to 0 | 30.4 to 22.6 | 86.7 (83.6 - 90.1) |
| RBBP8 | −7 to 0 | 33.5 to 25.8 | 87.6 (84.4 - 91.0) |
| STC2 | −7 to 0 | 32.1 to 23.9 | 81.2 (77.7 - 85.0) |
| UBE2C | −7 to 0 | 32.7 to 24.3 | 78.1 (74.4 - 82.2) |
| RPL37A | −7 to 0 | 26.6 to 18.7 | 85.3 (83.1 - 87.5) |
| HBBV2 | −6 to 0 | 32.7 to 25.6 | 79.0 (74.7 - 83.9) |
95% confidence intervals are indicated in brackets. For linear range a maximum deviation from linearity of 1 Ct value was accepted.
Kronenwett et al. BMC Cancer 2012 12:456 doi:10.1186/1471-2407-12-456
