Table 2

Overall variabilities and variabilities of the EP scores, Cqvalues, and normalized ΔCqvalues of all genes
Standard deviations
Cq ΔCq EP
variables
 day 0.024 0.013 0.006
 day time < 0.001 0.016 <0.001
 PCR instrument 0.027 0.009 0.037
 sample position 0.028 0.015 <0.001
 plate lot 0.014 0.011 <0.001
 reagent lot 0.085 0.027 <0.001
 operator 0.003 0.014 0.043
total variable noise 0.097 0.042 0.057
replicate noise 0.176 0.111 0.136
total noise 0.201 0.119 0.147

Different operating conditions (variable noise, replicate noise, and total noise) are indicated as standard deviations of the Cq values, ΔCq values, and the EP scores.

Kronenwett et al.

Kronenwett et al. BMC Cancer 2012 12:456   doi:10.1186/1471-2407-12-456

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