Table 1

LoB, LoD, linear dynamic range, PCR efficiency of the 12 PCR assays included in EndoPredict
Gene LoB LoD [Cqvalue] Linear range [log2dilution step] Linear range [Cqvalue] Efficiency [%]
AZGP1 40.0 35.6 (34.2 - 36.4) −13.1 to 0 35.6 to 20.5 81.9 (80.3 - 83.6)
CALM2 40.0 35.4 (34.3 - 36.0) −14.0 to 0 35.4 to 21.6 101.4 (99.8 - 103.2)
BIRC5 40.0 36.3 (35.4 - 36.9) −9.1 to 0 36.3 to 26.7 93.3 (90.5 - 96.2)
DHCR7 40.0 36.3 (35.4 - 36.8) −10.9 to 0 36.3 to 24.5 89.9 (87.6 - 92.3)
IL6ST 40.0 36.8 (35.7 - 37.5) −11.5 to 0 36.8 to 23.3 80.7 (78.8 - 82.7)
MGP 40.0 37.2 (35.2 - 38.2) −13.9 to 0 37.2 to 20.2 76.3 (74.4 - 78.2)
OAZ1 40.0 36.6 (35.5 - 37.2) −12.9 to 0 36.6 to 22.6 89.0 (87.6 - 90.4)
RBBP8 40.0 35.6 (34.7 - 36.1) −9.4 to 0 35.6 to 25.9 96.3 (93.2 - 99.6)
STC2 40.0 35.1 (34.2 - 35.7) −9.9 to 0 35.1 to 24.0 85.2 (82.6 - 87.9)
UBE2C 40.0 36.0 (34.9 - 36.7) −10.1 to 0 36.0 to 24.4 83.3 (81.1 - 85.7)
RPL37A 40.0 36.0 (34.5 - 36.7) −16.4 to 0 36.0 to 19.0 94.5 (92.9 - 96.1)
HBBV2 40.0 35.3 (33.6 - 36.2) −7.6 to 0 35.3 to 25.9 75.4 (70.1 - 81.6)

95% confidence intervals are indicated in brackets. For linear range a maximum deviation from linearity of 1 Ct value was accepted.

Kronenwett et al.

Kronenwett et al. BMC Cancer 2012 12:456   doi:10.1186/1471-2407-12-456

Open Data