Stimulation of NK cells in the presence of monocytes and PstS-1. Purified NK cells (5 x 105 cells/well) were admixed to monocytes (NK + Mo) in 1:1 cell-ratio and cultured with or without PstS-1 (10 μg/ml) for one and three days. NK cells were examined for the expression of CD69 on NK cells by flow cytometry on day one and three (a), the IFN-γ-release by ELISA (b) and the cytotoxicity against five ovarian cancer cell lines in the CD107a-degranulation-assay on day three (c). Means + SE of three to six independent experiments for each cell line are shown. Statistical analysis was performed by unpaired t-test, statistical significance was assumed at p ≤ 0.05.
Gottschalk et al. BMC Cancer 2012 12:451 doi:10.1186/1471-2407-12-451