Figure 4.

Differential resolution of γH2A.X foci in proliferating and quiescence-enriched KG1a cells. (a) Evaluation of cellular RNA content after treatment with 100nM rapamycin for 48 hours. (i) Flow cytometric dotplots indicating the percentage of cells which are low in RNA (Pyronin Y) as well as low in DNA (7-AAD); (ii) Mean and standard deviation of 5 independent assays; (iii) The total RNA content of lysed cells, measured by spectrophotometry (mean and standard deviation of 3 independent assays). (b) Proliferating and quiescence-enriched KG-1a cells were treated with 3 μM daunorubicin for 2 hours. Cells were then washed twice in ice cold RPMI at 4°C and were re-suspended in fresh culture medium and placed back in the incubator to allow a 2 hour repair period. γH2AX foci were measured by immunohistochemistry using the H score as described. Values correspond to the mean +/− standard deviation for the increase in γH2A.X foci compared with untreated controls, n = 3

Jawad et al. BMC Cancer 2012 12:431   doi:10.1186/1471-2407-12-431
Download authors' original image