Replication of MV-CEA in human HB cells. CEA level of the (a) intracellular and (b) culture supernatant in the Hep2G cell line was detected by ELISA from 24 to 96 hours after infection at an MOI of 0.1. The intracellular CEA levels increased with time and peaked at 72 hours post infection, and the supernatant CEA level peaked at 84 hours post infection in Hep2G cells but not in L-02 cells. This phenomenon provides strong evidence for MV-CEA replication and cell lysis.
Zhang et al. BMC Cancer 2012 12:427 doi:10.1186/1471-2407-12-427