Additional File 3 .

Hypoxic enhancement of exosome release as detected by CD63 immunoblot. (A) CD63 and CD9 immunoblot of SKBR3 ExoquickTM precipitants from a 48 hour culture under normoxia or 1% O2, including band intensity quantitation. (B) CD63 immunoblot of SKBR3 ExoquickTM precipitants from a 24 hour culture under normoxia or 0.1% O2, including band intensity quantitation. All CD63 immunoblots were performed under non-reducing conditions as described previously [16]. (PDF 29 kb).

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King et al. BMC Cancer 2012 12:421   doi:10.1186/1471-2407-12-421