Additional File 1 .

Impact of hypoxia on cell growth and viability. (A, B) MCF7, SKBR3 and MDA-MB 231 breast cancer cells were cultured for 48 hours under normoxia or 1% O2. Cell counts were performed for each well after hypoxic exposure (A) and cell viability was determined by Trypan blue exclusion (B) (n=4; ± SEM). (C, D) MCF7, SKBR3 and MDA-MB 231 breast cancer cells were cultured for 24 hours under normoxia or 0.1% O2 and cell counts (C) and viability (D) data were obtained as described above (n=4; ± SEM). ** corresponds with P value < 0.01. (PDF 13 kb).

Format: PDF Size: 14KB Download file

This file can be viewed with: Adobe Acrobat Reader

King et al. BMC Cancer 2012 12:421   doi:10.1186/1471-2407-12-421