Table 1

Primer sequences and annealing temperatures for direct sequencing
Gene Exon Primer sequence Tm,
PIK3CA 9 F,5’-TTGCTTTTTCTGTAAATCATCTGTG-3’ 51
R,5’-CCACAAATATCAATTTACAACCATTG-3’
20 F,5’-GGTATTAACATCATTTGCTCCAA-3’ 52
R,5’-CCTATGCAATCGGTCTTTGC-3’
KRAS 1 F,5’-CATTACGATACACGTCTGCAGTCAACTGG-3’ 52
R,5’-GTGAACATCATGGACCCTGACATACTCC-3’
BRAF 15 F,5’-TCATAATGCTTGCTCTGATAGGA-3’ 52
R,5-GGCCAAAATTTAATCAGTGGA-3’

F, forward primer R, reverse primer Tm, annealing temparature.

We used DNA sequencing to detect mutations in the clinical HNSCC specimens. KRAS (exons 1 and 2), BRAF (exon 15) and PIK3CA (exons 9 and 20) were analyzed for mutations.

Suda et al.

Suda et al. BMC Cancer 2012 12:416   doi:10.1186/1471-2407-12-416

Open Data