Table 1 |
|||
| Primer sequences and annealing temperatures for direct sequencing | |||
| Gene | Exon | Primer sequence | Tm, |
| PIK3CA | 9 | F,5’-TTGCTTTTTCTGTAAATCATCTGTG-3’ | 51 |
| R,5’-CCACAAATATCAATTTACAACCATTG-3’ | |||
| 20 | F,5’-GGTATTAACATCATTTGCTCCAA-3’ | 52 | |
| R,5’-CCTATGCAATCGGTCTTTGC-3’ | |||
| KRAS | 1 | F,5’-CATTACGATACACGTCTGCAGTCAACTGG-3’ | 52 |
| R,5’-GTGAACATCATGGACCCTGACATACTCC-3’ | |||
| BRAF | 15 | F,5’-TCATAATGCTTGCTCTGATAGGA-3’ | 52 |
| R,5-GGCCAAAATTTAATCAGTGGA-3’ | |||
F, forward primer R, reverse primer Tm, annealing temparature.
We used DNA sequencing to detect mutations in the clinical HNSCC specimens. KRAS (exons 1 and 2), BRAF (exon 15) and PIK3CA (exons 9 and 20) were analyzed for mutations.
Suda et al. BMC Cancer 2012 12:416 doi:10.1186/1471-2407-12-416
