Figure 2.

Differential effects of Hsp90 inhibition in primary cells and in cancer cells lines. a) Quantitative mapping of kinase populations in Hs68, U2OS, SW480 and A549 cells after geldanamycin treatment at two time intervals. Protein level changes over time at 12 h and 24 h were analyzed and kinases with similar behaviour were grouped as defined in the main text. Pie charts show for each cell line the relative proportion of respective groups compared to all quantified kinases. Log2 ratio fc (treated versus untreated cells) of -0.4,-0.8 and 0.4 were used as thresholds. b) Many kinases are strongly decreased in cancer cells when compared to non-transformed Hs68 cells. Kinase log2 ratio fc (24 h treated versus untreated cells) of cancer cells are compared to those of Hs68 cells. Differences of more than -0.5 were considered as differential and the graph shows kinases with an effect in at least two cell lines. c) Kinases from heat map data from Figure 1A are grouped according to KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway classification. Candidate Hsp90 clients are in bold. Delta average of the log2 ratio (24 h treated versus untreated) and the respective percentage difference between each cancer cell line and Hs68 is displayed. fc - fold change.