Open Access Highly Accessed Research article

Hsp90 inhibition differentially destabilises MAP kinase and TGF-beta signalling components in cancer cells revealed by kinase-targeted chemoproteomics

Armin Haupt1, Gerard Joberty2, Marcus Bantscheff2, Holger Fröhlich23, Henning Stehr1, Michal R Schweiger1, Axel Fischer1, Martin Kerick1, Stefan T Boerno1, Andreas Dahl1, Michael Lappe1, Hans Lehrach1, Cayetano Gonzalez45, Gerard Drewes2 and Bodo MH Lange1*

Author Affiliations

1 Department of Vertebrate Genomics, Max-Planck Institute for Molecular Genetics, Ihnestrasse 73, 14195 Berlin, Germany

2 Cellzome AG, Meyerhofstrasse 1, 69117 Heidelberg, Germany

3 International Center for Information Technology, University of Bonn, 53113 Bonn, Germany

4 Cell Division Group, IRB-Barcelona, PCB, c/Baldiri Reixac 10-12, 08028 Barcelona, Spain

5 Institucio Catalana de Recerca i Estudis Avançats, Passeig Lluís Companys 23 08010 Barcelona, Spain

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BMC Cancer 2012, 12:38  doi:10.1186/1471-2407-12-38

Published: 25 January 2012

Additional files

Additional file 1:

Supplementary Methods. Detailed methods for experimental design, mass spectrometry, statistical analyses and next generation sequencing.

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Additional file 2:

Supplementary_Table 1. Detailed results of the kinobeads-MS experiments with geldanamycin treatment.

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Additional file 3:

Supplementary_Table 2-5. Table 2 summarizes quantification data and indicates known clients including references for interactions and Hsp90 inhibitor effects. Table 3 lists protein level changes of heat shock proteins after geldanamycin treatment for 24 h. Table 4 lists kinetics of individual kinases in all four cell lines. Table 5 differential quantification of kinases when compared to Hs68 following 24 h treatment with geldanamycin.

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Additional file 4:

Supplementary_Figure 1. Shown are the western blot results of two experiments comparing DMSO and geldanamycin treatment for 24 h.

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Additional file 5:

Supplementary References. Reference list for literature cited in Supplementary Table 2.

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Additional file 6:

Supplementary_Table_6. Detailed results of the kinobeads-MS experiments with combined geldanamycin and MG132 treatment.

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Additional file 7:

Supplementary_Table_7. Results of the sequencing of 144 kinase genes in Hs68, SW480, U2OS and A549 cells.

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Additional file 8:

Supplementary_Documents_Description. Description of each supplementary table content and supplementary figure description.

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