Inhibition of miR-21 mediated apoptosis in both mouse and human tumor cells. A) AnnexinV staining showing that both mouse and human glioma cells undergo apoptosis upon miR-21 inhibition by using LNA-miR-21 as compared to si-control. B) A panel of mouse glioma cell lines (left), the human glioblastoma primary culture U3001MG (center), and the human glioblastoma cell lines Cl2:6 and U2987MG (right) showed an increased level of cleaved Caspase-3 after LNA-miR-21 as compared to si-control and two independent miRNAs (miR-29a and miR-29b) as shown by Western blot analysis. GAPDH was used as a loading control.
Põlajeva et al. BMC Cancer 2012 12:378 doi:10.1186/1471-2407-12-378