Down-regulation of NDRG2 enhanced cisplatin-induced apoptosis of Hela cells. Hela cells and the stable variants were exposed to different concentration of cisplatin (CDDP) for 24 h. The apoptotic cells were either stained with annexin V-FITC and propidium iodide (PI) and examined with flow cytometry, or subjected to Western blot. A. The percentage of apoptotic cells was presented. # p < 0.05 vs Hela and scramble control. * p < 0.01 vs Hela and scramble control. B. The expression of caspase-3 (Casp-3) and caspase-9 (Casp-9) was detected by Western blot analysis using β-actin as loading control. Shown are representative of 3 independent experiments. Act, active caspase. Pro, procaspase.
Liu et al. BMC Cancer 2012 12:370 doi:10.1186/1471-2407-12-370