Open Access Research article

Overexpression of cell cycle regulator CDCA3 promotes oral cancer progression by enhancing cell proliferation with prevention of G1 phase arrest

Fumihiko Uchida1, Katsuhiro Uzawa23*, Atsushi Kasamatsu3, Hiroaki Takatori4, Yosuke Sakamoto3, Katsunori Ogawara3, Masashi Shiiba3, Hideki Tanzawa23 and Hiroki Bukawa15

Author Affiliations

1 Department of Oral and Maxillofacial Surgery, Clinical Sciences, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan

2 Department of Clinical Molecular Biology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan

3 Division of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan

4 Department of Molecular Genetics, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan

5 Department of Oral and Maxillofacial Surgery, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8575, Japan

For all author emails, please log on.

BMC Cancer 2012, 12:321  doi:10.1186/1471-2407-12-321

Published: 28 July 2012

Additional files

Additional file 1:

mRNA and protein expression in shCDCA3-transfected cells using qRT-PCR and Western blot analyses. A, B) qRT-PCR shows that CDCA3 is down-regulated in shCDCA3-transfected cells compared with the mock-transfected cells (*p < 0.05, Mann-Whitney’s U test). Data are expressed as the means ± SEM of triplicate results. C, D) The densitometric CDCA3 protein levels in shCDCA3- and mock-transfected cells show that CDCA3 protein is markedly decreased in shCDCA3-transfected cells compared with mock-transfected cells (*p < 0.05, Mann-Whitney’s U test).

Format: TIFF Size: 888KB Download file

Open Data

Additional file 2:

Quantification of CDKIs (p21Cip1, p27Kip1, p15INK4B, and p16INK4A), CDK4, CDK6, Cyclin D1, and Cyclin E protein expression in shCDCA3- and mock-transfected cells. A, B) Western blot analysis shows up-regulation of p21Cip1, p27Kip1, p15INK4B, p16INK4A, CDK4, and Cyclin D1, and down-regulation of CDK6 and Cyclin E in the CDCA3 knockdown cells.

Format: TIFF Size: 1.1MB Download file

Open Data

Additional file 3:

Quantification ofWee1mRNA expression in OSCC-derived cell lines by qRT-PCR analysis. Significant down-regulation of Wee1 mRNA is seen in six OSCC-derived cell lines compared with the HNOKs (*p < 0.05, Mann-Whitney’s U test). Data are expressed as the means ± SEM of triplicate results.

Format: TIFF Size: 583KB Download file

Open Data

Additional file 4:

Quantification ofWee1mRNA expression in shCDCA3- and mock-transfected cells by qRT-PCR analysis. Significant up-regulation of Wee1 mRNA is seen in shCDCA3-transfected H1 (A) and Sa3 (B) cells compared with the mock-transfected cells (*p < 0.05, Mann-Whitney’s U test). Data are expressed as the means ± SEM of triplicate results.

Format: TIFF Size: 677KB Download file

Open Data