Additional file 1.
Figure S1. E2 induced the upregulation of bcl-2, cyclinD1 and survivin and moderately suppressed the level of the miRNAs in T47D cells. (A) T47D cells were incubated with phenol red-free IMEM supplemented with 5% charcoal stripped FBS for 48 h. Then cells were treated with vehicle control or 10 nM E2 for 24 and 48 h, total protein was extracted to detect the expression of bcl-2, cyclin D1 and survivin by Western blot. (B) RNA was extracted from the cells and RT-QPCR was used to examine the level of the miRNAs.
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Yu et al. BMC Cancer 2012 12:29 doi:10.1186/1471-2407-12-29