MiRNAs inhibit E2-induced cell proliferation. MCF-7 cells were transfected with 40 nM miR-16, miR-143 and miR-203 and treated with 10 nM E2 for 5 days. (A) MTS assay was used to detect cell proliferation. * denotes P < 0.05 compared with vehicle control. (B) MCF-7 cells were transfected with 40 nM miR-16, miR-143 and miR-203 and treated with 10 nM E2 for 48 h. Western blot was used to detect bcl-2, cyclin D1 and survivin expression. (C) The densitometry of each gene vs. β-actin was indicated. In negative control miRNA group, E2 significantly induced upregulation of bcl-2, cyclin D1 and survivin compared with vehicle control (* denotes P < 0.05). (D) In each miRNA-transfected group, the expression of bcl-2, cyclin D1 and survivin induced by E2 was normalized by that of vehicle control (ethanol). The fold change was statistically analyzed compared with negative control miRNA group. * denotes P < 0.05 compared with control.
Yu et al. BMC Cancer 2012 12:29 doi:10.1186/1471-2407-12-29