Overall structural analysis of GIG47 by CD and NMR techniques. (A) A CD spectrum of GIG47 (20 μM) obtained under 20 mM sodium acetate (pH6.5), 0.1 mM PMSF, 0.01 mM EDTA, 1 mM DTT, 0.1 mM benzamidine, 0.02% (w/v) NaN3 at 20°C. Low ellipticity at 220 nm indicates that the protein is mostly unstructured. (B) An 15 N-1 H HSQC spectrum of GIG47 (500 μM) obtained under 20 mM sodium acetate-d3 (pH 6.5), 0.1 mM PMSF, 0.01 mM EDTA, 1 mM DTT, 0.1 mM benzamidine, 0.02% NaN3, 90% H2O/10% 2H2O at 30°C with assigned resonances indicated.
Han et al. BMC Cancer 2012 12:274 doi:10.1186/1471-2407-12-274